High Sensitivity – Equipped with a 150 W xenon lamp and a high‑sensitivity photomultiplier tube (PMT), the detector delivers exceptional sensitivity, with a water Raman S/N ratio of ≥4000 (baseline method, 30 nm emission bandwidth), enabling trace‑level analysis.
Flexible Wavelength Configuration – Offers independently settable excitation (200–850 nm) and emission (250–900 nm) wavelengths, with programmable wavelength‑time switching for multi‑wavelength detection during a single run.
Dual Optical Bandwidth – Provides selectable emission spectral bandwidths (15 nm standard / 30 nm wide) to balance sensitivity and spectral resolution according to application needs.
Intelligent Maintenance & GLP Compliance – Features built‑in energy checks, wavelength accuracy verification using a mercury lamp, and comprehensive logbooks (Xe and Hg lamp cumulative ON time, number of ignitions, and replacement date records) to support GLP compliance and preventive maintenance.
Versatile Control Options – Supports full control via Kromstation chromatography data workstation (E‑LINE communication) or an optional keypad for standalone operation, with time‑programmable parameter changes (Ex, Em, PMT voltage, baseline) for automated method development..
| Parameter | Specification / Range |
| Optical System | Spectrophotometry; monitors emitted beam intensity |
| Light Source | 150 W Xenon lamp; Mercury (Hg) lamp for wavelength calibration |
| Excitation Wavelength Range | 200 – 850 nm (zero order available) |
| Emission Wavelength Range | 250 – 900 nm (zero order available; photomultiplier must be changed for emission >731 nm) |
| Wavelength Accuracy | ±3 nm |
| Wavelength Repeatability | ≤ ±0.5 nm |
| GLP Support | Lamp energy check, wavelength accuracy check, lamp ON time/replacement records |
| Communication | e Line (digital) |
| Processor Output | 1 V full scale (optional); digital via e-Line |
| Flow Cell | Standard 12 µL (irradiated capacity) |
| Flow Cell Pressure Rating | 1.0 MPa |
| Operating Temperature | 4 – 35 °C (non condensing) |
| Dimensions (L × W × H) | 470 mm × 340 mm × 300 mm |
| Weight | 25 kg |
| Power Requirements | 100 – 240 VAC, 50/60 Hz |
| Power Consumption | 250 W |
The EClassical D3251L Fluorescence Detector delivers high-sensitivity, low-noise fluorescence detection for HPLC systems, supporting both routine and challenging analytical applications. It enables precise qualitative and quantitative analysis of fluorescent compounds across a wide wavelength range (Ex: 200–850 nm, Em: 250–900 nm). The detector provides programmable wavelength-time switching and time-programmed parameter changes (Ex, Em, PMT voltage, baseline) for automated method optimization. With selectable emission bandwidths (15 nm or 30 nm) and multiple time constants, it can be tailored to specific sensitivity and resolution requirements. Built-in GLP functions—including lamp energy checks, wavelength accuracy verification, and lamp logbooks—ensure data integrity and simplified maintenance. It can be fully controlled via Kromstation workstation or operated independently with an optional keypad. With a water Raman S/N ≥4000 (30 nm bandwidth), the D3251L is well-suited for trace analysis in pharmaceutical, environmental, food safety, and life science applications.
