The two-dimensional high-performance liquid chromatograph (HPLC) is an advanced separation and analytical instrument that enhances traditional liquid chromatography by incorporating a second-dimensional separation system. This enables sample separation across two distinct dimensions, significantly improving separation capacity and resolution. It is particularly suitable for analyzing complex samples, such as biological samples, natural products, and drug metabolites.
The working principle of a two-dimensional high-performance liquid chromatography (HPLC) system is based on two distinct separation mechanisms. The first dimension typically employs reversed-phase chromatography, which separates compounds according to their hydrophobicity. The second dimension may utilize hydrophilic interaction chromatography, ion exchange chromatography, or other complementary mechanisms to further separate the components eluted from the first dimension.
E3200L 2D-LC system employs patented pulse damping technology and a dual-pump system to achieve stable delivery and rapid switching of the mobile phase. The entire system supports six-column temperature control to ensure reproducibility of the separation process. Samples are introduced into the first chromatographic column via an autosampler, where the separated components are eluted and transferred to the second chromatographic column for further separation, ultimately being detected by the detector.
To cater to diverse application scenarios and client needs, the E3200L2D-LC platform by E-Lite offers multiple configurations and system types, designed to deliver flexible and comprehensive solutions.
Full-spectrum PDA type: Equipped with a photodiode array detector, capable of simultaneous 190-800 nm full-wavelength scanning during separation. Suitable for method development, unknown substance identification, peak purity testing, and complex research requiring acquisition of compound ultraviolet spectral information.
Multi-detector combination: It can be connected with differential refractometer, fluorescence detector or evaporation light scattering detector simultaneously or optionally, which greatly expands the range of compounds to be detected and is suitable for the analysis of carbohydrates, lipids, and compounds with no or weak ultraviolet absorption.
Preparation and purification system: Based on two-dimensional analysis, it can be coupled with a fraction collector to achieve automated, high-resolution preparation and purification based on two-dimensional separation. Particularly suitable for the separation and preparation of active components in natural products, synthetic impurities, proteins, and peptides.
Online Pre-treatment-Analytical Integration System: This system integrates online solid-phase extraction (SPX) and online dilution derivatization modules, achieving full automation from sample purification, enrichment to two-dimensional separation and analysis. It significantly reduces human error and enhances reproducibility, making it particularly suitable for direct analysis and high-throughput screening of biological samples (e.g., plasma, urine).
Customized dedicated analytical systems: Deeply tailored for specific industries and applications, such as proteomics analysis systems, traditional Chinese medicine fingerprinting analysis systems, and polymer additive analysis systems. Both hardware and software are optimized for specific separation mechanisms and data processing requirements.
