The S3220L Auto-sampler features a simultaneous movement of tray and sample needle that shortens injection cycles and supports a wide range of tray formats (72‑, 96‑, 120‑, 210‑well and double 96‑well plates). An imported high‑precision side‑opening sample needle made of high‑purity special steel enhances strength, reduces adsorption, and eliminates blockage from septum debris. A high‑precision stepper motor combined with world‑class guide rails achieves positioning accuracy close to “zero deviation”. The highly integrated 32‑bit STM32 microcontroller with SPI/I²C bus minimizes board size and improves reliability. Intelligent features include fully automatic power‑on self‑test, dual‑probe leakage alarm, and software‑controlled lighting. The instrument offers three injection modes (full loop, partial loop with sample loss, partial loop without sample loss) and built‑in automatic dilution and derivatization functions, enabling unattended completion of the entire sample preparation and injection process. Both inner and outer walls of the injection probe are cleaned, achieving sample residue below 0.005%.
| Parameter | Specification / Range |
| Injection Range | 0.1 – 100 µL (ES3220L); 0.1 – 20 µL (ES3221L/ES3222L) |
| Injection Repeatability (RSD) | < 0.2% (full loop) < 0.5% (partial loop, lossy) < 1.0% (partial loop, lossless) |
| Injection Linearity | r² > 0.999 |
| Sample Residue | < 0.005% |
| Maximum Pressure | 45 MPa (standard), 102 MPa (optional), 120 MPa (optional) |
| Communication | TCP (Ethernet) |
| Injection Trigger | Digital mode, analog mode |
| Cleaning Method | Inner and outer wall cleaning |
| Cleaning Solvent Selection | Supports strong/weak cleaning solvent selection |
| Leakage Alarm | Dual leakage sensors |
| Sample Tray Compatibility | 72‑, 96‑, 120‑, 210‑well, double 96‑well trays |
| Power Requirements | AC 220 V ± 10%, 50 Hz |
| Power Consumption | 80 W |
| Fuse Rating | T2AL250V |
| Dimensions (W × D × H) | 600 mm × 380 mm × 180 mm |
| Weight | 16 kg |
The S3220L Auto-sampler is designed for ultra-high-performance liquid chromatography (UHPLC) systems, providing fully automated, high-precision sample introduction with built-in dilution and derivatization capabilities. It autonomously executes the entire workflow—from sample dilution and chemical derivatization to injection—without operator intervention, dramatically reducing time and eliminating manual errors. Three selectable injection modes ensure optimal performance for various analytical needs, delivering excellent repeatability (RSD < 0.2%), superb linearity (r² > 0.999), and ultra‑low sample carryover (< 0.005%). The dual‑wall cleaning mechanism and optional high-pressure tolerance (up to 120MPa) make it ideal for demanding UHPLC applications. Seamlessly integrated via TCP communication with the Kromstation chromatography workstation, the S3220L enables complete remote control, method programming, and real-time monitoring, ensuring reliable, unattended operation for pharmaceutical, biomedical, environmental, and industrial QC laboratories requiring high throughput and uncompromised data quality.