Elite ACO Carbamate pesticides Analysis Column

The Elite ACO column has good selectivity for 10 types of carbamate pesticides and excellent separation effect. The chromatographic column and detection method exhibit good repeatability, linear correlation, and accurate quantitative results.

Good separation effect：The Elite ACO chromatographic column, coupled with stable gradient elution, exhibits excellent separation performance, enabling the effective separation of 10 carbamate pesticides.

The system exhibits excellent stability, with 7 consecutive sample injections showing a retention time RSD value of ≤1.0% for 10 carbamate pesticides, and a peak area RSD value of ≤3%.

The pre-treatment method is simple and reliable, utilizing the QuEChERS method for sample processing. It is fast, straightforward, with high recovery rates and stability.

High sensitivity: Post-column derivatization fluorescence detection is adopted, resulting in high detection sensitivity and minimal interference from impurities.

Carbamate pesticides all carry a methylamino group -NHCH3 in their structure, which can be hydrolyzed to methylamine under the action of alkali solution. Methylamine can react with orthophthalaldehyde and β-mercaptoethanol to form a strong fluorescent substance, 1-(2-hydroxyethyl)thio-2-methylisoindole.

In this method, carbamate pesticides are first separated by a chromatographic column, then alkali-hydrolyzed into methylamine in a post-column derivatization device, and finally detected using a fluorescence detector after derivatization. This method is highly selective, with less matrix interference, and is one of the most effective and sensitive methods for detecting carbamate pesticides.

Step 1: Hydrolysis

Step 2: Derivation