The Elite-AAP amino acid analysis column has good selectivity for all 17 kinds of amino acids and excellent separation effect.
The dedicated reversed-phase C18 amino acid analysis column has excellent selectivity for all 17 kinds of amino acids, and the system separation effect is good. Equipped with a guard column, it effectively extends the service life of the chromatographic column and reduces the analysis cost.
The Elite-AAP amino acid analysis system adopts the pre-column derivatization method. Through a high-precision binary gradient high-performance liquid chromatography system, it provides precise control of mobile phase components. It is equipped with a dedicated C18 bonded stationary phase to achieve excellent separation of 17 amino acids, and the detection of amino acids is completed by a UV detector.
Free amino acids in beer
Sample processing: The sample was filtered through a 0.45μm or 0.22μm filter membrane, and 200µL of the filtrate was taken for derivatization.
Free amino acids in soy sauce
Sample processing: Take 0.5mL of the sample, add 2mL of acetonitrile, vortex for 20 seconds, and centrifuge (10,000 RPM) for 15 minutes.
Take the supernatant and conduct derivatization
Analysis principle
The most commonly used method for amino acid analysis at present is to react amino acids with derivatization reagents containing hydrophobic groups before separation to form compounds that are conducive to retention and separation on a reversed-phase column. After column separation, qualitative and quantitative analysis is carried out through the corresponding detector, which is the pre-column derivatization method.
The Elite-AAP amino acid analysis system uses phenyl isothiocyanate (PITC) as the pre-column derivatization reagent. Under alkaline conditions, amino acids react with PITC to form phenylamino thioformamide (PTC) derivatives with ultraviolet absorption, which are then separated and detected.
This derivatization method is not only simple to operate, but also ensures stable derivatization products, and provides accurate qualitative and quantitative analysis of samples. Its disadvantages are that the derivatization reagents have an impact on the lifespan of the chromatographic column, and the linearity of cystine is not good.
Amino acid derivatization reaction equation
the standard fractions of the 18 basic amino acids
1. Asp aspartic acid; 2. Glu glutamic acid; 3. Ser serine; 4. DNP-OH 2, 4-dinitrophenol; 5. Arginine 6. Gly glycine; 7. Thr threonine 8. Pro proline 9. Ala alanine; 10. Val valine; 11. Met methionine; 12.Cys cysteine; 13. Ile isoleucine; 14. Leucine 15. Trp tryptophan; 16. Phe phenylalanine; 17. Histidine 18. DNFB 2, 4-dinitrofluorobenzene; 19.Lys lysine; 20. Tyr tyrosine
| Name | Specification | P/N |
|---|---|---|
| Elite AAP Column | 4.6×250 | 31110163-C |
| Amino acid reagent kit | 31990026 | |
| Reference standards | 17 amino acids | 31990024 |