Amber Sugar H5/8/10 5/10µm

The Amber Sugar columns can be used for the separation of small biological organic molecules such as carbohydrates, organic acids, peptides, and cellular metabolites.

Amber Sugar H8 Column: Features sulfonic acid groups (-SO₃H) bonded to the surface of 8% cross-linked polystyrene/divinylbenzene (PS/DVB) particles. It is primarily used for the detection of fermentation products, fruit juices containing organic acids, alcohols, and sugars.

The uniform particle size ensures high resolution and efficient separation.
Crosslinking degrees of 5%, 8%, and 10% are available.
The mobile phase uses pure water or dilute acid, making the operation simple and with good reproducibility.
The selection range of particle morphology is wide: H+, Ca2+, Pb2+, K+, Na+.
The inter-batch reproducibility is good.
Good durability

Specification

Specification		Amber Sugar H8	Amber Sugar Ca8	Amber Sugar Pb8	Amber Sugar K8	Amber Sugar Na8
Matrix		PS/DVB	PS/DVB	PS/DVB	PS/DVB	PS/DVB
Particle Size (μm)		5,10	5,10	5,10	5,10	5,10
Cross-linking Degree		5%,8%,10%	5%,8%,10%	5%,8%,10%	5%,8%,10%	5%,8%,10%
Stationary Phase		-SO₃ H	-(SO₃ )₂Ca	-(SO₃ )₂Pb	-SO₃ K	-SO₃Na
pH Stability		1-3	5-9	5-9	5-9	5-9
Maximum Pressure (psi)	10 μm	1000	1000	1000	1000	1000
Maximum Pressure (psi)	5 μm	800	800	800	800	800
Recommended Mobile Phase		2.5mM H₂SO₄OR 0.1% H₃PO₄	H₂O	H₂O	H₂O	H₂O
Recommended Flow Rate (mL/min)		0.4-0.8	0.4-0.8	0.4-0.8	0.4-0.8	0.4-0.8
Maximum Temperature (°C)		85	85	85	85	85

Application

Organic Acids

Column: Amber Suger H8 ( 5 µm, 8%, 7.8 x 300 mm )
Mobile phase: 2.5 mM H2SO4
Flow rate: 0.5 mL/min
Temperature: 55℃
Detection: RI(35 ℃)
Injection volume: 50 µL

Sodium citrate

Sample Category: Food additive
Separation Mode: Ion-exchange chromatography
Column: Amber Suger H10: 8% ( 10 µm, 7.8 x 300 mm )
Mobile phase: 5mM H2SO4
Flow rate: 0.3 mL/min
Detection: UV210nm
Injection volume: 10 µL
Sample: 2mM Sodium Citrate by water

Trehalose

Sample Category: Biochemistry
Separation Mode: Ion-exchange chromatography
Column: Amber Suger H5 ( 10 µm,5%, 7.8 x 300 mm)
Mobile phase: Water
Flow rate: 0.4 mL/min
Temperature: 80 ℃
Detection: RI 45℃
Injection volume: 20 µL
Sample: 1. Maltotriose ( 2.5 mg/mL ) 2. Trehalose ( 10 mg/mL ) 3. Glucose ( 2.5 mg/mL )

Glucose、Succinic acid、Lactic acid、Formic acid、Acetic acid

Sample Category: Chemical industry
Separation Mode: Ion-exchange chromatography
Column: Amber Suger H8 ( 10 µm,8%, 7.8 x 300 mm)
Mobile phase: 2.5 mM H2SO4
Flow rate: 0.5 mL/min
Temperature: 55 ℃
Detection: RI ( 35 ℃ )
Injection volume: 20 µL
Sample: 1.Glucose ( 2.0 mg/ml ), 2.Succinic acid ( 0.05 mM ), 3.Lactic acid ( 0.1 mM ), 4.Formic acid ( 0.1 mM ), 5.Acetic acid (0.1 mM )

Vibarivin

Column: Amber Suger H8 ( 10 µm,8%, 7.8 x 300 mm)
Mobile phase: Water
Flow rate: 1.0 mL/min
Temperature: 55 ℃
Injection volume: 20 µL
Detection: UV207nm

How It Works

The Amber Sugar series of chromatographic columns is specially designed for the high-efficiency separation and preparation of water-soluble or partially water-soluble organic compounds, and is applicable to the separation of bio-organic small molecules in saccharides, organic acids, polypeptides and cellular metabolites.

Structural schematic diagram of

Various Ion Forms

The availability of multiple selectable ion forms for Amber Sugar resins enables the Amber Sugar series columns to be widely used for the separation of sugar molecules, sugar alcohols and other water-soluble substances in various matrices. For example, arabinose, ribitol and arabitol are difficult to separate on H-form columns, yet achieve baseline separation on Ca-form columns; xylose, galactose and mannose elute as an overlapping peak on Ca-form columns, while they can be eluted sequentially as individual peaks on Pb-form columns.

Table 1 Selection of Different Ion Forms

Ion	Application
H	Fermentation products, fruit juices containing organic acids, alcohols and saccharides
Ca	Saccharides in high-fructose corn syrup; especially suitable for the separation of monosaccharides, disaccharides, trisaccharides, tetrasaccharides and sugar alcohols
Pb	Pentoses and hexoses in wood products; dairy products containing sucrose, lactose, etc.
K	Sucrose, honey, corn syrup, beet sugar and other sugar-containing plant products.
Na	Oligosaccharides, especially in the presence of a high concentration of inorganic sodium ions (e.g., honey)

Excellent Stability

The superior manufacturing process and advanced packing technology of Amber Sugar columns ensure their high stability. The resin remains stable in pure water and other aqueous buffer systems even under elevated temperature conditions.

Ordering Information

Name	Size(mm)	P/N
Amber Sugar H5 10μm	7.8×300	3111-8b26k-g81-C
Amber Sugar H8 10μm	7.8×300	3111-8b26m-g81-C
Amber Sugar H10 10μm	7.8×300	3111-8b26n-g81-C