Amber SEC80/100/150/300 Plus 3µm

The Amber series represents ELITEHPLC's latest size exclusion chromatography columns, including the Amber SEC series for aqueous phases and the Amber GPC series for organic phases.

Amber SEC

Amber SEC columns are produced using patented surface modification technology, which involves bonding a uniform, nanoscale-thick neutral hydrophilic film onto a high-purity silica gel matrix with excellent mechanical stability. The process employs controlled chemical modification technology, ensuring complete coverage of the hydrophilic coating and reliable column-to-column reproducibility. This series of columns not only exhibits exceptional stability but also demonstrates minimal nonspecific adsorption for biological samples such as proteins. The carefully designed large pore volume ensures high separation capacity and excellent resolution, making it widely applicable for the separation and detection of biomolecules and water-soluble polymers.

Silica gel with surface-bonded hydrophilic film
Wide range of pore size options
High capacity and high resolution
High protein recovery rate
Long service life
Suitable for multidimensional chromatography applications
Suitable for protein and enzyme analysis
Withstands temperatures up to 80°C
High stability in most aqueous buffers and organic solvents

Specification

Name	Particle Size (μm)	Pore Size (Å)	Molecular Weight Exclusion Range	Molecular weight range of water-soluble polymers	pH Range	Salt concentration range
Amber SEC 80 Plus	3	80	100 ‒ 50,000	500 ‒ 5,000	2 ‒ 8.5	20 mM - 2.0 M
Amber SEC 100 Plus	3	100	100 - 100,000	500-10,000	2 ‒ 8.5	20 mM - 2.0 M
Amber SEC 150 Plus	3	150	500 - 150,000	500-25,000	2 ‒ 8.5	20 mM - 2.0 M
Amber SEC 300 Plus	3	300	5,000 ‒ 1,250,000	1,000-100,000	2 ‒ 8.5	20 mM - 2.0 M

Application

1)Thyroglobulin, 670 kD; 2)γ-Globulin, 158 kD;3)Ovalbumin, 44 kD; 4)Myoglobin, 17.6 kD;5)Poly-DL-alanine(1-5 kD); 6)B12,1.35 KD.

Chromatogram of mixed protein separation on Amber SEC Plus 300

Column: Amber SEC Plus 300 , 3 µm, 7.8 x 300 mm
Mobile phase: 150 mM Sodium phosphate buffer, pH=7.0
Flow rate: 1.0 mL/min
Temperature: Ambient
Detection: UV 214nm
Injection volume: 10 µL

Monoclonal antibody separation on Amber SEC Plus 300

Column: Amber SEC 150 Plus, 3 µm, 7.8 x 300 mm
Mobile phase: 150 mM Phosphate buffer ( pH 7.0)
Isopropanol=97: 3(v/v)
Flow rate: 1.0 mL/min
Temperature: Ambient
Detection: UV 214nm
Injection volume: 10 µL

How It Works

This bonded stationary phase is prepared using 3 μm high-purity silica gel as the matrix, with a uniform, nanoscale neutral hydrophilic coating covalently bonded to the silica gel surface via a patented surface modification technology. The adoption of a controlled chemical modification technology ensures reliable batch-to-batch reproducibility of the packing material. The Amber SEC Plus packing utilizes a chemical bonding process that achieves full coverage of the hydrophilic coating on the surface. Thus, it not only exhibits exceptional stability but also shows extremely low non-specific adsorption toward biological samples such as proteins. Meanwhile, its large pore volume ensures a high sample loading capacity and outstanding resolution

Protein molecular weight calibration curve for Amber SEC Plus

Unique stationary phase structure

Differences in stationary phase modification

Ordering Information

P/N	Name	Size	Molecular Weight Exclusion Range	Applicable Solvents
3111-80c32-g81-C	Amber SEC80 plus 3μm	ID7.8*300mm	500 ‒ 5,000（water-soluble polymers） 100-50,000(proteins)	Conventional Aqueous and Organic Solvents
3111-80c34-g81-C	Amber SEC100 plus 3μm	ID7.8*300mm	500-10,000（water-soluble polymers） 100-100,000(proteins)
3111-80c3c-g81-C	Amber SEC150 plus 3μm	ID7.8*300mm	500-25,000（water-soluble polymers） 500-150,000(proteins)
3111-80c39-g81-C	Amber SEC300 plus 3μm	ID7.8*300mm	1,000-100,000（water-soluble polymers） 5,000-1,250,000(proteins)