Amber SEC80/100/150/300 3µm

The Amber series represents Elite's latest size exclusion chromatography columns, including the Amber SEC series for aqueous phases and the Amber GPC series for organic phases.

Amber SEC

Amber SEC columns are produced using patented surface modification technology, which involves bonding a uniform, nanoscale-thick neutral hydrophilic film onto a high-purity silica gel matrix with excellent mechanical stability. The process employs controlled chemical modification technology, ensuring complete coverage of the hydrophilic coating and reliable column-to-column reproducibility. This series of columns not only exhibits exceptional stability but also demonstrates minimal nonspecific adsorption for biological samples such as proteins. The carefully designed large pore volume ensures high separation capacity and excellent resolution, making it widely applicable for the separation and detection of biomolecules and water-soluble polymers.

Silica gel with surface-bonded hydrophilic film
Wide range of pore size options
High capacity and high resolution
High protein recovery rate
Long service life
Suitable for multidimensional chromatography applications
Suitable for protein and enzyme analysis
Withstands temperatures up to 80°C
High stability in most aqueous buffers and organic solvents

Specification

Name	Particle Size (μm)	Pore Size (Å)	Molecular Weight Exclusion Range	Molecular weight range of water-soluble polymers	pH Range	Salt concentration range
Amber SEC 80	3	80	100 ‒ 50,000	500 ‒ 5,000	2 ‒ 8.5	20 mM - 2.0 M
Amber SEC 100	3	100	100 - 100,000	500-10,000	2 ‒ 8.5	20 mM - 2.0 M
Amber SEC 150	3	150	500 - 150,000	500-25,000	2 ‒ 8.5	20 mM - 2.0 M
Amber SEC 300	3	300	5,000 ‒ 1,250,000	1,000-100,000	2 ‒ 8.5	20 mM - 2.0 M

Application

Enoxaparin Sodium for Injection

Column: Amber SEC 300 , 3 µm, 7.8 x 300 mm
Mobile phase: 0.5M LiNO<sub>3</sub>
Flow rate: 0.6 mL/min
Temperature: 25℃
Detection: RI（35℃）
Injection volume: 20 µL

Recombinant human growth hormone

Column: Amber SEC 150, 3 µm, 7.8 x 300 mm
Mobile phase: 0.06 M Phosphate buffer ( pH 7.0)
Isopropanol=97: 3(v/v)
Flow rate: 0.6mL/min
Temperature: 25℃
Detection: UV214nm
Injection volume: 20 µL

E. coli lysate(2.5 mg/mL)

Column: Amber SEC 150, 3 µm, 7.8 x 300 mm
Amber SEC 300, 3 µm, 7.8 x 300 mm
Mobile phase: 0.15 M Phosphate buffer ( pH 7.0)
Flow rate: 1.0 mL/min
Detection: UV280nm
Injection volume: 10 µL

- PEG-modified BSA
  
  Column: Amber SEC 300, 3 µm, 7.8 x 300 mm
  
  Mobile phase: 150 mM Phosphate buffer ( pH 7.0)
  
  Flow rate: 1.0 mL/min
  
  Detection: UV280nm
  
  Temperature: 25℃
  Sample: BSA control (5mg/mL in Sodium acetate),10 μLPEG 20 kD,10 mg/mL,5μ L20 kD Pegylated BSA,4.23 mg/mL,12μL

How It Works

The Amber SEC size exclusion chromatography column uses 3 μm monodisperse spherical silica gel as the packing material, with a nanoscale neutral hydrophilic film covalently bonded to its surface. Surface bonding technology ensures maximum surface functionalization of the packing, endowing it with high chemical stability and extremely low non-specific adsorption.

Excellent Features
3 μm particle size packing material
Ultra-high column efficiency and resolution
Consistent batch-to-batch reproducibility
High protein recovery with minimal protein denaturation
Extremely low non-specific adsorption

This column is suitable for the separation and preparation of conventional biological samples, including proteins, nucleic acids, oligonucleotides, polypeptides and viruses. It is also applicable for the analysis and purification of neutral polymers, including polysaccharides, synthetic polymers and nanomaterials.

Protein molecular weight calibration curve for Amber SEC

Ordering Information

P/N	Name	Size	Molecular Weight Exclusion Range	Applicable Solvents
3111-80b32-g81-C	Amber SEC80 3μm	ID7.8*300mm	500 ‒ 5,000（water-soluble polymers） 100-50,000(proteins)	Conventional Aqueous and Organic Solvents
3111-80b34-g81-C	Amber SEC100 3μm	ID7.8*300mm	500-10,000（water-soluble polymers） 100-100,000(proteins)
3111-80b3a-g81-C	Amber SEC150 3μm	ID7.8*300mm	500-25,000（water-soluble polymers） 500-150,000(proteins)
3111-80b39-g81-C	Amber SEC300 3μm	ID7.8*300mm	1,000-100,000（water-soluble polymers） 5,000-1,250,000(proteins)