The Amber series represents ELITEHPLC's latest size exclusion chromatography columns, including the Amber SEC series for aqueous phases and the Amber GPC series for organic phases.
Amber SEC columns are produced using patented surface modification technology, which involves bonding a uniform, nanoscale-thick neutral hydrophilic film onto a high-purity silica gel matrix with excellent mechanical stability. The process employs controlled chemical modification technology, ensuring complete coverage of the hydrophilic coating and reliable column-to-column reproducibility. This series of columns not only exhibits exceptional stability but also demonstrates minimal nonspecific adsorption for biological samples such as proteins. The carefully designed large pore volume ensures high separation capacity and excellent resolution, making it widely applicable for the separation and detection of biomolecules and water-soluble polymers.
| Name | Particle Size (μm) | Pore Size (Å) | Molecular Weight Exclusion Range | Molecular weight range of water-soluble polymers | pH Range | Salt concentration range |
|---|---|---|---|---|---|---|
| Amber SEC 100 Plus | 5 | 100 | 100 - 100,000 | 500-10,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 150 Plus | 5 | 150 | 500 - 150,000 | 500-25,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 300 Plus | 5 | 300 | 5,000 ‒ 1,250,000 | 1,000-100,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 500 Plus | 5 | 500 | 15,000 - 5,000,000 | 2,500-500,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 1000 Plus | 5 | 1000 | 50,000 - 7,500,000 | 5,000-1,500,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 2000 Plus | 5 | 2000 | > 10,000,000 | 50,000->2,500,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
Excellent stability
The bonded stationary phase of Amber SEC Plus is prepared via a patented surface modification technology, which involves bonding a uniform neutral hydrophilic coating with nanoscale thickness to a high-purity silica gel matrix. Thanks to the adoption of a controlled chemical modification technology, reliable batch-to-batch reproducibility of the packing material is ensured. The Amber SEC Plus packing utilizes a chemical bonding technology, and its surface is fully covered with a hydrophilic coating. Thus, it not only exhibits excellent stability but also shows extremely low non-specific adsorption toward biological samples such as proteins. Meanwhile, the large pore volume ensures a high sample loading capacity and outstanding resolution.
Protein molecular weight calibration curve for Amber SEC Plus
Thyroglobulin, 670 kD; 2. γ -Globulin, 158 kD;3. BSA, 66 kD; 4. Ovalbumin, 44 kD5. Myoglobin, 17.6 kD; 6. Ribonuclease A, 13.7 kD..7.B12,1.35kD;8.Uracil, 120D
Column: Amber SEC Plus 300, 5 µm, 7.8 x 300 mm
Mobile phase:150 mM Sodium phosphate buffer ( pH 7.0 )
Flow rate: 1.0mL/min
Detection: UV 214 nm
| P/N | Name | Size | Molecular Weight Exclusion Range | Applicable Solvents |
|---|---|---|---|---|
| 3111-80c54-g81-C | Amber SEC100 plus 5μm | ID7.8*300mm | 500-10,000（water-soluble polymers） 100-100,000(proteins) | Conventional Aqueous and Organic Solvents |
| 3111-80c5a-g81-C | Amber SEC150 plus 5μm | ID7.8*300mm | 500-25,000（water-soluble polymers） 500-150,000(proteins) | |
| 3111-80c59-g81-C | Amber SEC300 plus 5μm | ID7.8*300mm | 1,000-100,000（water-soluble polymers） 5,000-1,250,000(proteins) | |
| 3111-80c5b-g81-C | Amber SEC500 plus 5μm | ID7.8*300mm | 2,500-500,000（water-soluble polymers） 15,000-5,000,000(proteins) | |
| 3111-80c5d-g81-C | Amber SEC1000 plus 5μm | ID7.8*300mm | 5,000-1,500,000（water-soluble polymers）50,000-7,500,000(proteins) | |
| 3111-80c5f-g81-C | Amber SEC2000 plus 5μm | ID7.8*300mm | 50,000->2,500,000（water-soluble polymers） ＞10,000,000(proteins) |