The Amber series represents ELITEHPLC's latest size exclusion chromatography columns, including the Amber SEC series for aqueous phases and the Amber GPC series for organic phases.
Amber SEC columns are produced using patented surface modification technology, which involves bonding a uniform, nanoscale-thick neutral hydrophilic film onto a high-purity silica gel matrix with excellent mechanical stability. The process employs controlled chemical modification technology, ensuring complete coverage of the hydrophilic coating and reliable column-to-column reproducibility. This series of columns not only exhibits exceptional stability but also demonstrates minimal nonspecific adsorption for biological samples such as proteins. The carefully designed large pore volume ensures high separation capacity and excellent resolution, making it widely applicable for the separation and detection of biomolecules and water-soluble polymers.
| Name | Particle Size (μm) | Pore Size(Å) | MW exclusion range | MW range of water-soluble polymers | pH range | Salt concentration range |
|---|---|---|---|---|---|---|
| Amber SEC 100 | 5 | 100 | 100 - 100,000 | 500-10,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 150 | 5 | 150 | 500 - 150,000 | 500-25,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 300 | 5 | 300 | 5,000 ‒ 1,250,000 | 1,000-100,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 500 | 5 | 500 | 15,000 - 5,000,000 | 2,500-500,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 1000 | 5 | 1000 | 50,000 - 7,500,000 | 5,000-1,500,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
| Amber SEC 2000 | 5 | 2000 | > 10,000,000 | 50,000->2,500,000 | 2 ‒ 8.5 | 20 mM - 2.0 M |
Excellent stability
The surface of the Amber SEC stationary phase is coated with an extremely dense hydrophilic, neutral polymer film, which prevents the mobile phase from contacting the inner side of the film and avoids film delamination, thus maintaining high stability even at high flow rates.
Column: Amber SEC-300, 5 µm, 7.8 x 300 mm
Mobile phase:150 mM Sodium phosphate buffer ( pH 7.0 )
Flow rate: 1.7mL/min
Sample: MAB
High protein recovery
The Amber SEC stationary phase is a neutral, hydrophilic stationary phase. There is almost no non-specific interaction between proteins, other biomolecules and the stationary phase of Amber SEC columns. Proteins do not adsorb to the surface of the silica gel matrix, thus SEC columns deliver high protein recovery while maintaining excellent protein activity. For example, the sample recovery rate exceeds 95% in the separation of acidic protein BSA and basic protein lysozyme.
| P/N | Name | Size | Molecular Weight Exclusion Range | Applicable Solvents |
|---|---|---|---|---|
| 3111-80b54-g81-C | Amber SEC100 5μm | ID7.8*300mm | 500-10,000（water-soluble polymers） 100-100,000(proteins) | Conventional Aqueous and Organic Solvents |
| 3111-80b5a-g81-C | Amber SEC150 5μm | ID7.8*300mm | 500-25,000（water-soluble polymers） 500-150,000(proteins) | |
| 3111-80b59-g81-C | Amber SEC300 5μm | ID7.8*300mm | 1,000-100,000（water-soluble polymers） 5,000-1,250,000(proteins) | |
| 3111-80b5b-g81-C | Amber SEC500 5μm | ID7.8*300mm | 2,500-500,000（water-soluble polymers） 15,000-5,000,000(proteins) | |
| 3111-80b5d-g81-C | Amber SEC1000 5μm | ID7.8*300mm | 5,000-1,500,000（water-soluble polymers）50,000-7,500,000(proteins) | |
| 3111-80b5f-g81-C | Amber SEC2000 5μm | ID7.8*300mm | 50,000->2,500,000（water-soluble polymers） ＞10,000,000(proteins) |