The Amber Protein A 20 μm chromatographic column is specially designed for the rapid and accurate quantification of monoclonal antibodies in cell line screening, as well as in the process optimization and quality control of upstream bioprocesses. The packing material is prepared by coupling PS/DVB particles with recombinant Protein A ligands, which bind to all immunoglobulins except IgG3 and Fc-containing fusion proteins. This packing material features excellent mechanical strength and can withstand high flow rates and high-pressure operations.
| Item | Specification |
|---|---|
| Resin Matrix (Particle Size, Pore Size) | Uniform PS/DVB (20 μm, 1000–2000 Å) |
| Immobilized Ligand | Recombinant Protein A |
| Column Dimensions (ID × Length) | Stainless Steel |
| Maximum Pressure | 200 bar |
| pH Range | 1.2-13.0 |
| Maximum Flow Rate | 5 mL/min |
| Recommended Flow Rate | 1-3 mL/min |
| CIP | 0.1 M NaOH |
| Binding Buffer pH | 6.6-7.5 |
| Lifetime | >2000 Injections |
| Standard Injection Volume | 10 μL |
| IgG Detection Concentration | 0.029-40 mg/mL* |
| LOD | 0.29 μg |
(*UV280nm: 0.029 -7.500mg/mL, UV300nm: 0.117-40.000mg/mL)
| Parameter | Details |
|---|---|
| Column | 20 μm, 2.1 mm×30 mm; |
| Binding Buffer | 50 mM Phosphate, 150 mM NaCl, pH 7.0 |
| Elution Buffer | 100 mM Glycine, pH 2.5 |
| Gradient | 0.00–0.10 min: Binding Buffer0.11–0.60 min: Elution Buffer0.60–2.00 min: Binding Buffer |
| Flow Rate | 1.0 mL/min |
| IgG Concentration | 0.029–30 mg/mL |
| Sample | 2.0 mg/mL IgG + Supernatant |
| Temperature | 23°C |
| Injection Volume | 10 μL |
| Detector | HPLC-UV@280nmHPLC-UV@280 nm and 300 nm |
Flow rate up to 3 mL/min
Total cycle (including equilibration) time < 0.5 min
UV 280 nm: 0.029 – 7.50 mg/mL, LOD 0.294 μg
UV 300 nm: 0.117 – 40.0 mg/mL
The SEM image of Amber Protein A resin shows uniformly distributed particles with a particle size of 20 μm and a particle size distribution of D90/D10 < 1.3.
| Name | Size(mm) | P/N |
|---|---|---|
| Amber Protein A 20um | 2.1×30 | 3111-8a19e-2a1-C |