Amber HIC Butyl

Hydrophobic Interaction Chromatography Columns
The hydrophobic interaction chromatography series includes four models: Amber HIC Ethyl 5μm, Amber HIC Propyl 5μm, Amber HIC Butyl 5μm, and Amber HIC Phenyl 5μm. These columns are specifically designed and developed for high-efficiency separation of proteins, oligonucleotides, and peptides, featuring high resolution, high column efficiency, and excellent reproducibility. The packing matrix consists of uniform spherical, highly cross-linked non-porous polystyrene/divinylbenzene (PS/DVB) material. Ethyl, propyl, butyl, and phenyl hydrophobic ligands are uniformly and densely bonded to the PS/DVB surface, respectively, resulting in four hydrophobic interaction chromatography columns with progressively increasing hydrophobicity. This series is particularly suitable for separating monoclonal antibodies (Mab), antibody-drug conjugates (ADC), and related protein fragments with hydrophobic differences.

• Monodisperse particle packing, featuring high column efficiency and high resolution
• Wide pH range: 2 ~ 12
• Especially suitable for the separation and analysis of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs)
• Maximum operating temperature: 80°C

• Rapid Antibody Titer Determination

Flow rate up to 3 mL/min

Total cycle (including equilibration) time < 0.5 min

• High Linear Response Range (up to 40 mg/mL)

UV 280 nm: 0.029 – 7.50 mg/mL, LOD 0.294 μg

UV 300 nm: 0.117 – 40.0 mg/mL

• Durability: > 2000 Injections
• Reproducibility: Excellent Batch-to-Batch Consistency
• Suitable for Protein Quantification in CHO Cells with Varying Contents
• Compatible with HPLC, UPLC and FPLC Systems

The SEM image of Amber Protein A resin shows uniformly distributed particles with a particle size of 20 μm and a particle size distribution of D90/D10 < 1.3.