Amber GPC 100/300/500/1000/MP 5µm

The Amber series represents ELITEHPLC's latest size exclusion chromatography columns, including the Amber SEC series for aqueous phases and the Amber GPC series for organic phases.

Amber GPC

Amber GPC columns are specifically designed for high-efficiency separation of synthetic polymers. Based on highly cross-linked polystyrene/divinylbenzene (PS/DVB) particles with extremely narrow particle and pore size distributions, it delivers uniform pore size distribution that ensures accurate linear correlation between retention time and molecular weight. The highly cross-linked porous particles exhibit exceptional chemical and physical stability, maintaining consistent molecular weight calibration curves and column efficiency even when switching organic solvents. With large pore volume, Amber GPC packing guarantees high resolution in polymer separations. The packing is available as either single-pore-size resin or blended resins with different pore sizes.

Matrix: Highly cross-linked polystyrene/divinylbenzene (PS/DVB) particles
Compatibility: Applicable to virtually all organic solvents (e.g., THF, DMAC, DMF, TCB, NMP, HFIP)
Max Temperature Resistance: 145°C
Uniform particle and pore size distribution
High pore volume and resolution
Low background noise in light scattering detection

Specification

Stationary Phase	Particle Size (μm)	Pore Size (Å)	MW exclusion range	pH Range	Maximum Pressure(psi)
Amber GPC 100	5	100	100 - 100,000	1-14	5000
Amber GPC 300	5	300	500-250,000	1-14	5000
Amber GPC 500	5	500	1,000-750,000	1-14	5000
Amber GPC 1000	5	1000	5,000-2,500,000	1-14	5000
Amber GPC-MP	5	100-1000	5,000-2,500,000	1-14	5000

Application

Polystyrene

Column: Amber GPC 500 ( 5 µm, 500 Å, 7.8 x 300 mm )
Mobile phase: DMF + 0.25 % TBAB
Flow rate: 0.8 mL/min
Detection: Refractive index ( 35℃ )
Injection volume: 20 µL
Column temperature: Room temperature
Sample: 10.0 mg/mL polystyrene ( 70 kD )

Cefterampivoxil

Column: Amber GPC 100 ( 5 µm, 100 Å, 7.8 x 300 mm )
Mobile phase: DMF + 20 mM LiBr
Flow rate: 0.5mL/min
Detection: UV263nm
Injection volume: 20 µL
Sample: 1 mg/mL Cefterampivoxil

1.Triglyceride Diacylglycerol 3. Monoglyceride

Glyceride in fish oil

Column: Amber GPC 300, 5 µm, 300 Å, 7.8 × 300 mm
Amber GPC 100, 5 µm, 100 Å, 7.8 × 300 mm
Mobile phase: THF
Flow rate: 1.0mL/min
Detection: Refractive index
Column temperature: Room temperature

How It Works

Matrix: Highly cross-linked polystyrene/divinylbenzene (PS/DVB) particles
Compatibility: Applicable to virtually all organic solvents (e.g., THF, DMAC, DMF, TCB, NMP, HFIP)
Max Temperature Resistance: 145°C
Uniform particle and pore size distribution
High pore volume and resolution
Low background noise in light scattering detection

In routine polymer analysis, the molecular weight of samples can reach 2,000,000 Daltons, and analytical and preparative separations can be performed under elevated temperature conditions.

Typical applications include: Polystyrene, polyolefin, polyacrylate, polysiloxane, polycarbonate, resins.

Polystyrene molecular weight calibration curve

Polystyrene molecular weight: 570Da,1920Da,3460Da,9580Da,17300Da,27500Da,62300Da,

139000Da,319000Da,601800Da,1103000Da,1760000Da,2860000Da

Ordering Information

P/N	Name	Size (mm)	Molecular Weight Exclusion Range	Applicable Solvents
3111-80a54-g81-C	Amber GPC100 5μm	ID7.8*300mm	100 - 100,000	Almost all organic solvents（Such as THF, DMAC, DMF, TCB, NMP, HFIP, etc.）
3111-80a59-g81-C	Amber GPC300 5μm	ID7.8*300mm	500-250,000
3111-80a5b-g81-C	Amber GPC500 5μm	ID7.8*300mm	1,000-750,000
3111-80a5d-g81-C	Amber GPC1000 5μm	ID7.8*300mm	5,000-2,500,000
3111-80a5h-g81-C	Amber GPC-MP 5μm	ID7.8*300mm	5,000-2,500,000